Accumulation of complex oligosaccharides and CAZymes activity under acid conditions constitute the Thatcher+Lr9 defence responses to Puccinia triticina

Abstract Puccinia triticina ( Pt ) is an important pathogen of wheat. While breeding programmes develop resistant wheat cultivars to mitigate the effects such rust-causing pathogens, emergence new rust races with wider virulence mandates implementation other control strategies. Our study investigated whether acidic pH conditions affected selected Carbohydrate-Active Enzymes (CAZymes) in Pt- inoculated Thatcher + Lr9 (IR) compared those found (IS) The β-glucosidase and amyloglucosidase activity levels significantly increased IR from 1 14 days post-inoculation (dpi). In contrast, invertase did not change relative at 7 dpi, but were reduced plants dpi. IS had higher all three hexose-producing enzymes under conditions. These enzyme activities could be produce hexose sugars required by advance infection. phenotypic analysis supported this view because leaf disease symptoms only visible plants. For cell wall loosening-related enzymes, displayed exoglucanase, xylanase peroxidase IS. liquid chromatography–mass spectrometry showed concentrations complex oligosaccharides Thus, we concluded that involved loosening conditions, while building-blocks for synthesizing barriers apprehend growth .